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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in many tumors, which ends up in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was found to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a crucial position in marketing mobile-cycle development within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, are described. Two extra splice variants were reported but comprehensive nucleotide sequence details just isn't nevertheless readily available.
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Blocking peptides are peptides that bind exclusively on the goal antibody and block antibody binding. These peptide usually contains the epitope acknowledged by the antibody.
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This manual outlines additional probable optimization of cellular and immunoassay parameters to ensure the best possible benefits are...
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This tutorial outlines additional probable optimization of cellular and immunoassay parameters to be sure the very best outcomes are...
Antibodies certain to the blocking peptide now not bind to the epitope within the focus on protein. By evaluating the staining with the blocked antibody vs . the antibody by yourself, one can see which staining is specific.
Target Info Cyclin E belongs for the very conserved cyclin loved ones, whose customers are characterised by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins perform as regulators of CDK kinases. Different cyclins exhibit distinctive expression and degradation designs which add on the temporal coordination of each and every mitotic function. Cyclin E forms a fancy with and functions being a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates with the G1-S section boundary and is also degraded as cells development via S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...
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